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Early and precise detection of disease-specific genetic biomarkers is critical for effective diagnosis, treatment monitoring, and personalized medicine. Conventional molecular diagnostic platforms such as PCR are sensitive but require laboratory infrastructure, skilled personnel, and longer turnaround times. In this work, we present an innovative electrochemical biosensing device designed for multiplex detection of DNA and RNA biomarkers associated with infectious diseases and cancer. The platform utilizes a nano spaced interdigitated electrode (IDE) system integrated with sequence-specific capture probes to simultaneously detect multiple genetic targets. Signal amplification is achieved through gold nanorod-tagged reporter probes, enabling ultra-sensitive attomolar-level detection. The device offers rapid, label-free, and highly specific analysis suitable for point-of-care applications. This versatile molecular diagnostic technology has strong potential for deployment in clinical settings, supporting rapid disease screening, precision oncology, and outbreak surveillance.